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The aim of this paper was to explore the relationship between energy metabolism and the meridian phenomenon. The manner of change of oxygen partial pressure in acupoints and no-acupoints of the urinary bladder meridian of goats was observed with a needle-type tissue oxygen tension sensor after the acupuncture effect was blocked by Ca2+ complexation with ethylenediamine tetraacetic acid (EDTA)-Na2. The results showed that: (1) the concentration of calcium ion in urinary bladder meridian acupoints was higher than that in no-acupoints (P<0.01 or P<0.05); (2) the tissue oxygen tension in urinary bladder meridian acupoints was higher than that in no-acupoints (P < 0.01); (3)the oxygen partial pressure in acupoints decreased significantly after injection with EDTA-Na2,compared with injection of either normal sodium or nothing (P<0.01); (4) the oxygen partial pressure in acupoints was higher than that in no-acupoints after EDTA-Na2 injection (P< 0.01). Acupuncture could reduce oxygen partial pressure in acupoints by regulating microcirculation and increasing metabolic levels.