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目的：探讨中国人低密度脂蛋白受体（ＬＤＬ—Ｒ）基因ＮｃｏＩ多态性及其与血脂含量的关系。方法：合成引物一对，用ＰＣＲ技术扩增ＬＤＬ—Ｒ基因外显子１８的特异片段，用限制性内切酶ＮｃｏＩ酶切，得Ｎ＋Ｎ＋、Ｎ＋Ｎ－、Ｎ－Ｎ－３种基因型。结果：沈阳地区１０２例健康汉族人的ＬＤＬ—Ｒ基因ＮｃｏＩ多态性Ｎ＋Ｎ＋占４４．１％、Ｎ＋Ｎ－占４５．１％、Ｎ－Ｎ－占１０．８％。Ｎ＋等位基因频率０．６６７，Ｎ－等位基因频率０．３３３。Ｎ＋Ｎ＋、Ｎ＋Ｎ－、Ｎ－Ｎ－３组的血清总胆固醇含量依次为４．７８ｍｍｏｌ／Ｌ、４．１６ｍｍｏｌ／Ｌ、３．６５ｍｍｏｌ／Ｌ，差异显著（Ｆ＝８．１７Ｐ＜０．０５）。结论：提示Ｎ＋等位基因可能与高胆固醇有关系
Objective: To investigate the NcoI polymorphism of Chinese low density lipoprotein receptor (LDL-R) gene and its relationship with blood lipid content. Methods: A pair of primers was synthesized. The specific fragment of exon 18 of LDL-R gene was amplified by PCR and was digested with restriction endonuclease NcoI to get N + N +, N + N- and N-N-3 genotypes. Results: The NcoI polymorphism of LDL-R gene in 102 healthy Han nationality in Shenyang was 44.1%. N + N accounted for 45.1% and N-N accounted for 10.8%. The N + allele frequency was 0.667 and the N-allele frequency was 0.333. The levels of total cholesterol in N + N +, N + N- and N-N-3 groups were 4.78mmol / L, 4.16mmol / L and 3.65mmol / L respectively, with significant difference (F = 8.17P <0.05). Conclusion: It suggests that the N + allele may be related to high cholesterol