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目的探讨Hedgehog(Hh)信号通路阻断对人胃癌细胞SGC-7901侵袭的影响及其可能的机制。方法免疫荧光检测Hh信号通路分子Shh和Gli1蛋白在SGC-7901细胞中的表达;用不同浓度(2.5、5、10μmol/L)的Hh信号通路特异性阻断剂环靶明作用SGC-7901细胞48 h后,Transwell小室试验检测SGC-7901细胞的侵袭能力,半定量RT-PCR检测SGC-7901细胞中Shh、Gli1和血管内皮生长因子(VEGF)基因mRNA的表达。结果 Shh和Gli1蛋白在SGC-7901细胞中高表达;环靶明能显著抑制SGC-7901细胞的侵袭,且呈剂量依赖性;Hh信号通路阻断后,SGC-7901细胞中Shh基因mRNA的表达水平无明显改变,Gli1和VEGF基因mRNA的表达水平显著下降。结论 Hh信号通路分子在SGC-7901细胞中高表达,阻断Hh信号通路可以抑制SGC-7901细胞的侵袭,可能是通过抑制Gli1下调VEGF起作用。
Objective To investigate the effect of Hedgehog (Hh) signaling pathway on the invasion of human gastric cancer cell line SGC-7901 and its possible mechanism. Methods Immunofluorescence was used to detect the expression of Shh and Gli1 proteins in SGC-7901 cells. The Hh signaling pathway-specific inhibitors (2.5, 5 and 10μmol / L) were used to target SGC-7901 cells After 48 h, the invasion ability of SGC-7901 cells was detected by Transwell chamber assay. The mRNA expression of Shh, Gli1 and VEGF in SGC-7901 cells was detected by semi-quantitative RT-PCR. Results The Shh and Gli1 protein were highly expressed in SGC-7901 cells. The targeting of ring protein could significantly inhibit the invasion of SGC-7901 cells in a dose-dependent manner. Shh mRNA expression in SGC-7901 cells was blocked by Hh signaling pathway No significant change, Gli1 and VEGF gene mRNA expression levels decreased significantly. Conclusion The Hh signaling pathway is highly expressed in SGC-7901 cells. Blocking the Hh signaling pathway can inhibit the invasion of SGC-7901 cells, which may be through inhibiting the down regulation of Gli1.