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目的:研究蛋白酪氨酸磷酸酶1B(PTP-1B)在2型糖尿病发病中的作用。方法:将34例患者分为对照组、BMI正常的2型糖尿病初诊组(CDM组)和肥胖或超重的2型糖尿病初诊组(ODM组),测定空腹血糖、空腹胰岛素等临床指标,并以Western印迹法测定内脏脂肪组织中PTP-1B的表达水平。结果:ODM、CDM组较对照组已存在明显的胰岛素抵抗,但前两者胰岛素抵抗程度无明显差异,PTP-1B表达水平在上述3组逐渐降低(36.53±14.82,11.57±1.89,3.39±0.94);并且校正年龄和性别后PTP-1B表达水平与BMI呈正相关(r=0.67,P<0.01)。结论:PTP1B表达升高参与了胰岛素抵抗的发生,与2型糖尿病的发病关系密切;但PTP-1B的表达不仅与胰岛素抵抗因素相关,同时还受肥胖等因素的影响。
AIM: To investigate the role of protein tyrosine phosphatase 1B (PTP-1B) in the pathogenesis of type 2 diabetes. Methods: Thirty-four patients were divided into control group, normal new type 2 diabetes mellitus group (CDM group) and obese or overweight type 2 diabetes mellitus group (ODM group), and fasting blood glucose and fasting insulin were measured. Western blotting was used to detect the expression of PTP-1B in visceral adipose tissue. Results: Compared with the control group, ODM and CDM had obvious insulin resistance, but there was no significant difference in insulin resistance between the two groups. The expression level of PTP-1B in the three groups decreased gradually (36.53 ± 14.82,11.57 ± 1.89,3.39 ± 0.94 ), And the positive correlation between PTP-1B expression and BMI (r = 0.67, P <0.01) after adjusting for age and sex. CONCLUSION: The increased expression of PTP1B is involved in the development of insulin resistance, which is closely related to the pathogenesis of type 2 diabetes. However, the expression of PTP-1B is not only related to insulin resistance but also influenced by factors such as obesity.