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目的研究凋亡抑制蛋白细胞型Fas相关死亡域样白介素-1β转换酶抑制蛋白(c-FLIP)在胆囊癌和胆囊炎组织中的表达差异,探讨c-FLIP在胆囊癌发生、发展中所起的作用。方法收集49例石蜡包埋的胆囊癌标本和30例慢性胆囊炎标本,用免疫组化SP法测定c-FLIP的表达,分析其表达的差异及c-FLIP表达与肿瘤分期、分化、淋巴结转移和性别的关系。结果c-FLIP在胆囊癌组织中的阳性表达率显著高于胆囊炎组织(85.71%vs26.67%,P<0.01);c-FLIP的表达与患者性别、胆囊癌的分化程度、TNM分期及有无淋巴结转移之间的关联无统计学意义(P均>0.05)。结论c-FLIP过度表达可能是细胞发生恶变、对抗T细胞的杀伤作用甚至产生免疫耐受或免疫逃逸中的重要环节,在胆囊癌的发生、发展中起重要作用。
Objective To study the difference of the expression of c-FLIP in patients with gallbladder carcinoma and cholecystitis, and to investigate the role of c-FLIP in the occurrence and development of gallbladder carcinoma Role. Methods 49 specimens of paraffin-embedded gallbladder carcinoma and 30 specimens of chronic cholecystitis were collected. The expression of c-FLIP was detected by immunohistochemical SP method. The expression of c-FLIP and the correlation between c-FLIP expression and tumor stage, differentiation, lymph node metastasis And gender relations. Results The positive expression rate of c-FLIP in gallbladder cancer tissues was significantly higher than that in cholecystitis tissues (85.71% vs26.67%, P <0.01). The expression of c-FLIP was correlated with the gender, gallbladder carcinoma differentiation, There was no significant correlation between lymph node metastasis and lymph node metastasis (all P> 0.05). Conclusions The overexpression of c-FLIP may be an important link in the malignant transformation of cells, the anti-T cell killing effect and even immune tolerance or immune escape, which play an important role in the occurrence and development of gallbladder cancer.