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目的阐明艾滋病病毒1型(HIV-1)感染急性期,HIV脱氧核糖核酸(DNA)在CD+4T淋巴细胞(简称CD4细胞)亚群中的分布特点。方法将来自北京佑安医院的男男性行为者(MSM)HIV感染急性期队列,根据病人感染后病情进展速度的不同,将病人分为两组:一组20例病人,病情进展迅速,CD4细胞计数在2年内降低到200个/μL以下,为CD4细胞低组;另外一组23例病人,病情进展较慢,CD4细胞计数一直维持在500个/μL,为CD4细胞高组。收集病人急性期外周血,分离CD4细胞亚群,实时聚合酶链反应(RT-PCR)检测亚群中HIV DNA。结果记忆性CD4细胞中的HIV DNA含量为(4199±317.4)拷贝/100万细胞,高于幼稚CD4细胞中的(75±12.1)拷贝/100万细胞(P=0.0117);幼稚CD4细胞中HIV-1DNA的含量,CD4细胞低组高于CD4细胞高组(P<0.01)。结论 HIV-1急性感染期幼稚CD4细胞中HIV-1DNA可能和疾病进展有关。
Objective To clarify the distribution of HIV DNA in the subpopulation of CD + 4T lymphocytes (CD4 cells) in the acute phase of HIV-1 infection. Methods Acute cohorts of HIV-infected men who have sex with men (MSM) from Beijing You’an Hospital were divided into two groups according to their speed of disease progression. A group of 20 patients progressed rapidly and CD4 The count decreased to less than 200 cells / μL within 2 years, which was the low CD4 cell count. In the other 23 patients, the progression was slow and the CD4 cell count remained at 500 cells / μL, the highest CD4 cell count. Peripheral blood was collected from patients during the acute phase, CD4 cell subsets were isolated and HIV DNA was detected by real-time polymerase chain reaction (RT-PCR). Results HIV DNA content in memory CD4 cells was (4199 ± 317.4) copies / 100 000 cells higher than (75 ± 12.1) copies / 100 000 cells in naive CD4 cells (P = 0.0117) -1 DNA content, CD4 cell low group than CD4 cell high group (P <0.01). Conclusion The HIV-1 DNA in naive CD4 cells during the acute phase of HIV-1 may be related to the progression of the disease.