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目的：了解缺氧缺血对脑组织氧合状态及细胞生物氧化的影响及其在脑损伤中的作用。方法：应用近红外光谱测定技术连续监测缺氧缺血新生鼠脑内氧合血红蛋白、脱氧血红蛋白、总血红蛋白及氧化型细胞色素aa3，并测定脑细胞线粒体最大呼吸速度、呼吸控制比。结果；缺氧缺血后脑内氧合血红蛋白的迅速降低，脱氧血红蛋白升高（P＜0．01），2h内求缓解，同时细胞色素aa3升高。线粒体呼吸功能受损，缺氧1h、2h及对照组间差异显著（P＜0．01）。结论：缺氧缺血对脑氧合状态及线粒体功能影响迅速、严重，可导致脑能量代谢衰竭，成为脑损伤的首发环节。
Objective: To understand the effect of hypoxic-ischemic injury on oxygenation status and cell biooxidation in brain tissue and its role in brain injury. Methods: The contents of oxyhemoglobin, deoxyhemoglobin, total hemoglobin and oxidized cytochrome aa3 in brain of hypoxic-ischemic neonatal rats were continuously monitored by near infrared spectroscopy. The maximal respiration rate and respiratory control ratio of mitochondria in brain cells were measured. Results After hypoxic-ischemic brain oxygenation of hemoglobin decreased rapidly, deoxy-hemoglobin increased (P <0.01), 2h to seek relief, while elevated cytochrome aa3. Mitochondrial respiratory dysfunction, hypoxia 1h, 2h and the control group difference was significant (P <0.01). CONCLUSION: Hypoxic-ischemic attacks have a rapid and severe effect on cerebral oxygenation status and mitochondrial function, leading to metabolic failure of brain energy and becoming the first step of brain injury.