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本文以常用的补铁剂FeSO_4做为对照,比较了FeSO_4与银耳多糖-Fe(Ⅲ)(简称TFS-Fe(Ⅲ)配合物对红细胞膜的损伤作用,并用邻苯三酚在碱性条件下自氧化产生的超氧阴离子自由基O_2~-作为外源性自由基的模型来研究 TFS-Fe(Ⅲ)对红细胞膜的保护作用。通过脂质过氧化物的检测,自旋标记 ESR波谱的测定,膜蛋白电泳分离和红细胞溶血率测定等方法来探讨 TFS-Fe(Ⅲ)对红细胞的作用。实验结果表明:FeSO_4能促进脂质产生脂质过氧化物而使细胞膜受损伤,而 TFS-Fe(Ⅲ)能降低膜的脂质过氧化反应,并能阻抑膜蛋白发生交联,维持膜的正常流动性。体外实验结果提示:TFS-Fe(Ⅲ)配合物对细胞膜无损伤能力,而且具有保护作用。
In this paper, the commonly used iron-supplying agent FeSO 4 was used as a control. The damage of erythrocyte membrane by FeSO 4 and Tremella Polysaccharide-Fe(III) complex (abbreviated as TFS-Fe(III) complex) was compared, and pyrogallol was used under alkaline conditions. The superoxide anion radical O_2~- generated by auto-oxidation was used as a model of exogenous free radicals to study the protective effect of TFS-Fe(III) on erythrocyte membrane.The spin-labeled ESR spectra were detected by lipid peroxide detection. The determination of membrane protein electrophoretic separation and determination of red blood cell hemolysis rate was used to investigate the effect of TFS-Fe(III) on red blood cells.The experimental results showed that FeSO 4 can promote the production of lipid peroxides and cause cell membrane damage, while TFS- Fe(III) can reduce the membrane lipid peroxidation reaction, and can inhibit the membrane protein from cross-linking and maintain the normal fluidity of the membrane.The in vitro experimental results suggest that the TFS-Fe(III) complex has no damage to the cell membrane. And it has a protective effect.