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一九七七年十一月,我们开始进行大豆子叶下胚轴片段诱导幼苗的研究,并成功地产生了绿色体。材料与方法以杂交大豆为主要研究材料。严格精选无病斑(紫、褐斑)种子,用自来水冲洗24小时,使其萌动。取出后,放在75%酒精中消毒2～3分钟,再放于0.1%氯化汞溶液中浸10～12分钟。然后,将这些种子在无菌的蒸馏水中刷洗数次。在无菌间里,采用常规无菌操作方法,用镊子剥开膨胀了的豆瓣。从每根胚轴上
In November 1977, we started our research on seedlings induced by hypocotyl segments of soybean cotyledons and successfully produced green bodies. Materials and Methods Hybrid soybean was the main research material. Strict selection of disease-free spots (purple, brown spots) seeds, tap water for 24 hours to germinate. After taking out, sterilize it in 75% alcohol for 2 ~ 3 minutes and then dip in 0.1% mercuric chloride solution for 10 ~ 12 minutes. The seeds are then scrubbed several times in sterile distilled water. In the sterile room, using conventional aseptic method, with tweezers to peel the expanded watercress. From each hypocotyl