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为了探讨滴鼻免疫不同表型的双价痢疾菌苗的免疫效果 ,应用FSM 2 117(Ipa )及FS 5 40 2 (Ipa+)、FS 5 411(Ipa+)、FS 5 414(Ipa+)、FS 5 416 (Ipa+) 5株菌苗株分别滴鼻免疫小鼠 ,4× 10 7CFU/只 ,间隔 14d ,3次免疫后第 7天收集血清和小肠、鼻咽、肺、阴道冲洗液 ,ELISA法检测其中特异性福氏、宋内LPSIgA和IgG抗体水平 ,发现滴鼻免疫能够诱生血清中的双价IgA、IgG抗体显著升高 ,Ipa+株较Ipa 株升高显著 (P <0 0 1)。同时诱生鼻咽、肺、小肠内特异性抗体升高 ,尤其是生殖道冲冼液内抗体升高极为明显。表明鼻粘膜免疫不仅诱导多个粘膜部位 (特别是生殖道 )的抗体反应 ,且能诱导系统免疫反应 ,是一个安全有效的免疫途径。研究中发现侵袭蛋白表达在鼻粘膜部位能够显著加强系统特异性抗体生成。
In order to investigate the immunological effects of intranasal immunization with different phenotypes of bivalent dysentery bacterins, the effects of FSM 2 117 (Ipa), FS 5 40 2 (Ipa +), FS 5 411 (Ipa +), FS 5 414 (Ipa +), FS 5 The mice inoculated with 5 strains of 416 (Ipa +) were intranasally immunized with 4 × 10 7 CFU per mouse for 14 days. Serum and small intestinal, nasopharyngeal, pulmonary and vaginal washes were collected on the 7th day after 3 immunizations. Among them, the levels of LPSIgA and IgG in specific Freunds and Songs were significantly higher than those in Ipa strains (P <0.01). It was found that intranasal immunization induced the double IgA and IgG antibodies in serum. At the same time induced nasopharyngeal, lung, small intestine-specific antibodies increased, especially in the reproductive tract flushing fluid antibody significantly increased. It shows that nasal mucosal immunity not only induces the antibody reaction of multiple mucosal sites (especially the genital tract), but also induces systemic immune response, which is a safe and effective immunization pathway. The study found that invasion of protein expression in the nasal mucosa can significantly enhance the system-specific antibody production.