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目的 :探讨甾体激素对C6细胞摄取甘氨酸快速作用的非基因组织机制。方法 :应用液体闪烁技术 ,通过检测C6细胞在加入了甾体激素和 /或其它试剂后摄入标记甘氨酸量的改变 ,确定甾体激素的作用。结果 :C6细胞高亲合力的甘氨酸依赖于钠离子和氯离子。皮质酮 ,孕酮 ,地塞米松可快速抑制这种摄取 ,雌二醇 ,脱氧皮质酮无显著的抑制作用 ,表明甾体激素作用有特异性。皮质酮的作用在 10 4 -8～ 10 -6 mmol/L范围内效应与浓度成正相关。皮质酮偶联牛血清蛋白后作用依然存在。RU38486 能部分阻断皮质酮的效应。细胞外液钙离子缺乏时皮质酮的作用基本消失。结论 :虽然皮质酮 ,孕酮 ,地塞米松神经胶质细胞和神经元摄取甘氨酸的快速作用不一样 ,但均是非基因组机制。
OBJECTIVE: To investigate the mechanism of non-genomic effects of steroid hormones on uptake of glycine by C6 cells. METHODS: The effect of steroid hormones was determined by using liquid scintigraphy by detecting changes in uptake of labeled glycine in C6 cells after the addition of steroid hormones and / or other agents. RESULTS: High affinity glycine in C6 cells was dependent on sodium and chloride. Corticosterone, progesterone, dexamethasone can quickly inhibit this uptake, estradiol, deoxycorticosterone no significant inhibitory effect, indicating that the role of steroid hormone specificity. The effect of corticosterone was positively correlated with the concentration in the range of 10 4 -8 ~ 10 -6 mmol / L. Corticosterone coupled bovine serum albumin effect still exists. RU38486 partially blocked the effects of corticosterone. The role of corticosterone disappeared when extracellular calcium was absent. CONCLUSION: Although the rapid effects of glycine uptake by corticosterone, progesterone, dexamethasone glial cells and neurons are different, they are all non-genomic mechanisms.