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本文对流动相中尿素及其增溶的高浓度β环糊精的行为对用反相高效液相色谱进行甲基苯巴比妥对映体分离时的保留行为及分离度的影响进行了系统地探讨。对尿素在使流动相中β环糊精增溶的同时使分析物的保留时间降低，并致使对映体分离度降低的现象进行了分析讨论并用数学模型加以解释。
In this paper, the mobile phase of urea and its solubilization of high concentrations of β-cyclodextrin behavior reversed-phase high performance liquid chromatography for phenanthrobarbital enantiomeric retention retention behavior and resolution Systematically explore. The urea in the mobile phase of β cyclodextrin solubilization while reducing the retention time of the analyte, and resulting in decreased enantiomeric separation were analyzed and discussed with the mathematical model to be explained.