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光敏核不育水稻（农垦５８Ｓ）是中国水稻研究中的一个重要发现，在水稻杂交育种上有巨大潜力。研究表明：农垦５８Ｓ的雄性不育性受光周期调控，光敏色素是育性转变过程中光周期反应的光受体，然而，鉴于高等植物中存在至少两种不同的光敏色素分子，并且它们同时存在于水稻中，人们对调节农垦５８Ｓ雄性不育过程的光敏色素分子种类及作用方式仍然不清楚。本文采用酶联免疫吸附测定法（ＥＬＩＳＡ），比较了不同光周期下农垦５８Ｓ和对照品种农垦５８叶片（光周期感受器官）中光敏色素Ｉ（ｐｈｙＡ）的含量。从农垦５８Ｓ的二次枝梗原基分化期开始，进行１０天的光周期处理。一组为短日照（ＳＤ），另一组为长日照（ＬＤ）。在第１０个暗期结束前，于暗绿光下收获每株水稻的最上部两片新展叶，立即保存在液氮中。样品在研钵中磨成液氮粉，然后加入提取液（含５０ｍｍｏｌ／ＬＴｒｉｓ－ＨＣＩ和０．２ｍｏｌ／Ｌ巯基乙醇，ｐＨ８．５）匀浆。粗提液经０．５％聚乙烯亚胺（ＰＥＩ）沉淀后，上清液供ＥＬＩＳＡ分析。以燕麦ｐｈｙＡ的多克隆抗体、单克隆抗体分别作ＥＬＩＳＡ的一抗和检测抗体。采用ＥＬＩＳＡ可以专一性地检测水稻ｐｈｙＡ（Ｔａｂｌｅ１）。几次独立的实验说明，光周期处理对ｐｈｙＡ含?
Photo-sensitive genic male sterile rice (Nongken 58S) is an important discovery in Chinese rice research and has great potential in hybrid rice breeding. The results showed that male sterility of Nongken 58S was controlled by photoperiod, while phytochrome was photoreceptor of photoperiod response during fertility transformation. However, given the existence of at least two different phytochromes in higher plants and their simultaneous presence In rice, the molecular species and the mode of action of phytochromes regulating male sterility of Nongken 58S are still unclear. In this paper, the enzyme-linked immunosorbent assay (ELISA) was used to compare the content of phyA in Nongken 58S and control cultivar Nongken 58 at different photoperiod. From the second branch of Nongken 58S primordial differentiation period, the photoperiod of 10 days. One was short-day (SD) and the other was long-day (LD). Before the end of the 10th dark period, the top two new leaves of each rice plant were harvested under dark green light and immediately stored in liquid nitrogen. The sample was ground into liquid nitrogen powder in a mortar, and then an extract (containing 50 mmol / L Tris-HCI and 0.2 mol / L mercaptoethanol, pH 8.5) was homogenized. After the crude extract was precipitated with 0.5% polyethyleneimine (PEI), the supernatant was subjected to ELISA analysis. The polyclonal antibody and the monoclonal antibody of oat phyA were used as the primary antibody and the detection antibody respectively. ELISA can be used to detect rice phyA (Table 1). Several independent experiments show that photoperiod processing phyA containing?