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①目的 探讨睫状神经营养因子 (CNTF)对两种谷氨酸 (Glu)离子型受体激动剂 (α 氨基羧甲基异哑恶唑丙酸 ,AMPA ;N 甲基 D 天冬氨酸 ,NMDA)激发的海马神经元内游离Ca2 + ([Ca2 + ]i)升高的作用。②方法 原代培养海马神经元 ,在有或无CNTF条件下用活细胞内荧光探针Fura 2 AM实时检测AMPA和NMDA引起海马神经元内 [Ca2 + ]i 变化的情况。③结果 AMPA和NMDA均可引起细胞内 [Ca2 + ]i 升高 ,并呈量效依赖性 ;CNTF可快速抑制NMDA引起的海马神经元内 [Ca2 + ]i升高 (t=2 .97～ 4 .86 ,P <0 .0 5 ) ,而对AMPA的作用无影响 (t =0 .2 2～ 0 .74 ,P >0 .0 5 )。④结论 CNTF可能通过与NMDA型受体相互作用而快速启动Ca2 + 信号的途径 ,保护因Glu引起的海马神经元损伤。AMPA型受体可能与CNTF的快速作用无关。
Objective To investigate the effect of ciliary neurotrophic factor (CNTF) on the activity of two glutamate (Glu) receptor agonists (α-aminocrimethoxazole, AMPA; N-methyl D aspartate, NMDA) -induced elevation of free Ca2 + ([Ca2 +] i) in hippocampal neurons. ② Methods Primary cultured hippocampal neurons were cultured with live Fura 2 AM with or without CNTF for real-time detection of [Ca2 +] i changes in hippocampal neurons induced by AMPA and NMDA. ③ Results Both AMPA and NMDA increased the intracellular [Ca2 +] i in a dose-dependent manner. CNTF could rapidly inhibit the increase of [Ca2 +] i in hippocampal neurons induced by NMDA (t = 2.97 ~ 4.86, P <0.05), but had no effect on AMPA (t = 0.22 ~ 0.74, P> 0.05). CONCLUSION: CNTF can rapidly activate the Ca2 + signal through the interaction with NMDA receptors and protect the hippocampal neurons from injury induced by Glu. AMPA receptors may not have a direct effect on CNTF.