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据每日新闻6月4日报道,阪大工学部岡田教授于十年前从尼龙工厂的废液中发现了一种新菌,它以尼龙低聚体这一非天然化合物为营养源,命名为“产黄菌属KI72株”。从此开始研究它为什么不分解天然物质,而只分解尼龙低聚体之谜。已知这种细菌本身能产生E_1,E_2酶,而这两种酶能分解非天然化合物。于是研究了有否发出制造E_1,E_2酶信息的DNA(基因)。首先确认在KI72株的细胞内有三个质体(环状基因),其中只有一个质体能产生E_1,E_2酶。其次把产生E_1,E_2酶的质体分成微小的断片,再将这些断片植入大肠菌质体内,核对并找到了细菌中决定产生E_1、
According to the Daily News on June 4, Professor Okada of Osaka University’s Engineering Department discovered a new strain of waste bacteria from the nylon factory ten years ago. It uses the non-natural nylon oligomer as nutrient source and named “Flavobacterium genus KI72 strain.” Since then began to study why it does not break down the natural material, but only break down the mystery of nylon oligomers. It is known that this bacterium produces E_1 and E_2 enzymes by itself, and these two enzymes can break down non-natural compounds. Then, it was investigated whether or not DNA (gene) for producing E 1 and E 2 enzyme information was issued. First, we confirmed that there are three plastids (circular genes) in KI72 cells, of which only one plastid can produce E 1 and E 2 enzymes. Second, the plasmids producing E_1 and E_2 enzymes were divided into tiny fragments, and these fragments were then implanted into coliforms, and the bacteria were determined to produce E_1,