两系杂交稻腋芽在母体与离体条件下的再生特性

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以两系杂交稻培矮6 4S/E32、培两优5 0 0为材料,采用留高桩和节插两种试验方法,对两组合各节位腋芽的再生特性进行了研究。结果表明:(1)头季稻齐穗期中部腋芽较长,齐穗到齐穗后2 0d各节腋芽伸长速度均慢,齐穗后2 0d到成熟期伸长速度均加快,但上位芽更快。(2 )中高节位腋芽对母体营养物质的依赖性较低节位腋芽大,且其营养供应优先于低节位腋芽。(3)母体条件下腋芽再生力强弱,由遗传因素、母茎营养条件及生态条件等共同决定;只有将高桩再生稻试验与节插试验相结合,方可客观评价各节位腋芽决定于遗传因素的再生力强弱。(4)头季稻节间全氮含量与相应节位再生率的关系,齐穗期到齐穗后10d呈显著正相关,齐穗后2 0d和成熟期呈负相关或相关性较小;齐穗期节间可溶性糖+淀粉总量高不能提高再生率;齐穗后10d至成熟期,一般节间淀粉含量与再生率呈负相关,可溶性糖含量及可溶性糖+淀粉总量与再生率呈正相关。表明头季稻灌浆前期各节间的全氮含量是决定相应节位腋芽再生率高低的主导因素,而灌浆中后期可溶性糖含量高、淀粉含量低以及两者总量高时再生率高,起主导作用的是可溶性糖含量。 Two lines of hybrid rice Pei’ai 6 4S / E32 and Peiliangyou 500 were used as experimental materials to study the regeneration characteristics of axillary buds at each node of the two combinations. The results showed that: (1) The axillary buds in the middle heading stage of the first heading paddy were longer than that in the middle heading stage, and the axillary buds elongations were slow at 20 days after heading from heading to full heading, while the elongation rate increased from 20 days after heading to maturity Bud faster. (2) Axillary buds of mid-high node had less dependency on maternal nutrients than axillary buds of node, and its nutrient supply took precedence over axillary buds of lower node. (3) The strength of axillary bud regeneration under maternal conditions was determined by the genetic factors, nutrition conditions and ecological conditions of parent stems. Only by combining the test of high-pile regenerative rice with the test of inserting section, we could objectively evaluate the axillary bud of each node The regenerative power of genetic factors. (4) The correlation between total nitrogen content and corresponding node regeneration rate in the first crop of rice was significantly positive correlation between heading stage and 10 days after heading stage, but there was a negative correlation or less correlation between heading stage and maturity stage after heading stage. The soluble sugar content and total starch content at heading stage did not improve the regeneration rate. During 10 days after heading, the internode starch content was negatively correlated with the regeneration rate. The content of soluble sugar and soluble sugar + starch and regeneration rate Was positively correlated. It is indicated that the total nitrogen content in the first grain filling stage of the first season is the dominant factor that determines the regeneration rate of axillary buds at the corresponding node, while the soluble sugar content is high, the starch content is low and the total regeneration rate is high Leading role is soluble sugar content.
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