论文部分内容阅读
聚合酶链反应－单链构象多态性（ＰＣＲ／ＳＳＣＰ）是根据核苷酸序列不同的单链ＤＮＡ，可形成不同构象，从而在不含变性剂的聚丙烯酰胺凝胶中泳动速度不同的原理，分离鉴定等长ＤＮＡ单链的方法。本文应用ＰＣＲ／ＳＳＣＰ方法检测了６０例无亲属关系个体白细胞抗原Ⅱ类基因（ＨＬＡ－ＤＯＡ１）５－上游调控区的等位基因多态性，并以ＰＣＲ直接测序证明每种ＳＳＣＰ带型都与不同位置单个碱基取代相关联。
Polymerase chain reaction - single strand conformation polymorphism (PCR / SSCP) is based on the different single-stranded DNA nucleotide sequence can be formed in different conformation, and thus non-denaturing polyacrylamide gel in different speed of migration The principle of separation and identification of isogenic DNA single-strand method. In this study, PCR-SSCP was used to detect the allelic polymorphism of 5-upstream regulatory region of HLA-DOA1 gene from 60 unrelated individuals. PCR-SSCP was used to detect the polymorphism of each SSCP band Associated with a single base substitution at different positions.