论文部分内容阅读
目的探讨安徽省主要传疟媒介中华按蚊拟除虫菊酯击倒抗性机制,并为进一步建立抗性检测分子方法提供基础。方法采用自行设计的引物扩增中华按蚊钠离子通道ⅡS4-S6节段基因序列,扩增产物双向测序,获得的基因序列与GenBank数据库进行比对。结果受试中华按蚊均扩增出约378 bp均一清晰条带。测序比对发现,在相对于冈比亚按蚊钠离子通道1 013和1 014位置发生突变:由TTG突变为TTT或TGT,即L/F或L/C突变。结论安徽省中华按蚊已发生针对拟除虫菊酯的击倒抗性突变。
Objective To explore the mechanism of down resistance of pyrethroids in Anopheles sinensis in Anhui Province, and to provide a basis for further establishment of molecular methods for resistance detection. Methods The primers designed by ourselves were used to amplify the ⅡS4-S6 segment sequence of the sodium channel of Anoplasma sinensis, and the products were amplified by two-dimensional sequencing. The obtained gene sequences were compared with the GenBank database. Results Anopheles sinensis amplified about 378 bp uniform clear bands. Sequencing revealed a mutation at positions 0133 and 1104 relative to the Anopheles gambiae sodium channel: a mutation from TTG to TTT or TGT, ie, a L / F or L / C mutation. Conclusion An anti-pyrethroid knock-down resistance mutation has occurred in Anopheles sinensis in Anhui Province.