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目的观察慢性疼痛对新生大鼠学习记忆功能及海马神经细胞粘附分子(NCAM)表达的影响,探讨海马NCAM表达在慢性疼痛影响学习记忆机制中的作用。方法10窝新生SD大鼠60只,每窝随机分为疼痛组和对照组(n=30)。疼痛组在出生后2d(P2)左脚掌皮下注射完全弗氏佐剂20μl,对照组注射生理盐水20μl。分别于出生后11d(P11)和出生后22d(P22)用免疫组化法检测海马CA3区和齿状回NCAM的表达,并从P22开始,进行为期8d的水迷宫测试,分析其空间学习记忆功能的改变。结果疼痛组在水迷宫测试的P22和出生后26d寻找水中隐藏平台的潜伏期长于对照组(P<0.05)。疼痛组海马CA3区和齿状回NCAM的表达在P11和P22均低于对照组(P<0.05)。结论完全弗氏佐剂炎性疼痛对新生大鼠的学习记忆功能有抑制作用,其机制可能与炎性疼痛抑制海马CA3区和齿状回NCAM表达有关。
Objective To observe the effects of chronic pain on learning and memory ability and the expression of neural cell adhesion molecule (NCAM) in hippocampus of neonatal rats, and to explore the role of NCAM expression in hippocampus in learning and memory mechanism of chronic pain. Methods Sixty neonatal SD rats were randomly divided into the pain group and the control group (n = 30). In the pain group, 20 μl of complete Freund’s adjuvant was injected subcutaneously into the left paw 2 d after birth, and the control group was injected with 20 μl of saline. The expression of NCAM in hippocampal CA3 region and dentate gyrus was detected by immunohistochemistry on postnatal day 11 (P11) and postnatal day 22 (P22) respectively. From the beginning of P22, the water maze test was conducted for 8 days to analyze the spatial learning and memory Functional changes. Results In the pain group, the latent period of looking for the hidden platform in water maze tests P22 and 26 days after birth was longer than that of the control group (P <0.05). The expression of NCAM in hippocampal CA3 area and dentate gyrus in the pain group was lower than that in the control group at P11 and P22 (P <0.05). Conclusion Complete Freund adjuvant inflammatory pain inhibits the learning and memory function of neonatal rats. The mechanism may be related to inflammatory pain inhibiting the expression of NCAM in hippocampal CA3 region and dentate gyrus.