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目的:探讨大鼠氟乙酰胺中毒后心肌损伤情况及乙酰胺联合前列地尔注射液对心肌损伤的保护作用。方法:将100只Wistar大鼠随机分为正常对照组(A组),氟乙酰胺染毒组(B组),乙酰胺治疗组(C组),乙酰胺+前列地尔注射液治疗组(D组),前列地尔注射液治疗组(E组)。观察染毒后不同时间点(4h、1d、3d、7d)心肌组织形态及超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量变化。结果:氟乙酰胺中毒大鼠心肌细胞呈局灶性或片状变性、坏死,伴不同程度炎性细胞浸润;损伤于第1天出现,第3天最重,D组、C组第7天开始出现肉芽组织。与C组、E组比较,D组心肌损伤范围小,程度轻,心肌MDA含量明显降低(P<0.01);3组SOD活性比较差异无统计学意义。结论:氟乙酰胺可致心肌细胞局灶性或片状变性、坏死,肌纤维溶解。乙酰胺联合前列地尔注射液对氟乙酰胺中毒所致心肌损伤具有保护作用,其机制可能与乙酰胺阻断毒物体内进一步发展、前列地尔注射液抗氧化作用有关。
OBJECTIVE: To investigate the myocardial injury induced by fluoroacetamide in rats and the protective effect of acetamide combined with alprostadil injection on myocardial injury. Methods: 100 Wistar rats were randomly divided into normal control group (group A), fluoroacetamide group (group B), acetamide treatment group (group C), acetamide + alprostadil injection group Group D), alprostadil injection group (Group E). The changes of myocardial tissue morphology, SOD activity and malondialdehyde (MDA) content at different time points (4h, 1d, 3d, 7d) were observed. Results: Fluoroacetamide intoxication rats focal muscle cells or focal degeneration, necrosis, with varying degrees of inflammatory cell infiltration; injury appeared on the first day, the third day the heaviest, D group, C group on the 7th day Began to appear granulation tissue. Compared with group C and group E, myocardial injury in group D was small and mild, and MDA content in myocardium was significantly decreased (P <0.01). There was no significant difference in SOD activity among the three groups. Conclusion: FFA can cause focal or lamellar degeneration of cardiomyocytes, necrosis and dissolution of muscle fiber. Acetamide combined with alprostadil injection could protect myocardial injury induced by fluoroacetamide, and its mechanism may be related to the further development of acetamide blockade in vivo and the antioxidation of alprostadil injection.