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目的:建立甲壳素胶囊中壳聚糖含量的测定方法。方法:采用OP(聚乙二醇辛基苯基醚)乳化剂荧光猝灭法。在pH4.5的B-R缓冲溶液中,波长为λex/λem=226/304nm时,考察壳聚糖对OP乳化剂的荧光猝灭作用。结果:壳聚糖检测浓度在0.20～2.00mg.L-1范围内与OP乳化剂的荧光猝灭程度呈线性关系(r=0.9993),检出限为0.039mg.L-1;平均加样回收率为96.90%,RSD=3.25%(n=6)。结论:该法简便、快速、准确可靠,可用于较为复杂样品中微量壳聚糖的含量测定。
Objective: To establish a method for determination of chitosan in chitin capsule. Methods: Fluorescence quenching method using OP (polyethylene glycol octylphenyl ether) emulsifier. In pH4.5 B-R buffer solution, the wavelength of λex / λem = 226 / 304nm, the effect of chitosan on OP emulsifier fluorescence quenching. Results: The linear relationship was found between the concentration of chitosan and OP in the range of 0.20 ~ 2.00 mg.L-1 (r = 0.9993), the detection limit was 0.039 mg.L-1. The recovery was 96.90%, RSD = 3.25% (n = 6). Conclusion: The method is simple, rapid, accurate and reliable and can be applied to the determination of trace chitosan in more complex samples.