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采用宽带激光熔覆技术,梯度设计的思想,添加不同含量稀土氧化物Nd2O3来提高复合涂层生物活性的方法,在TC4钛合金表面制备了含HA+β-TCP的稀土梯度生物活性陶瓷涂层。使用MG63人成骨细胞与Nd2O3活性梯度涂层体外共培养,用MTT法测定成骨细胞碱性磷酸酶含量和通过SEM观察MG63细胞在活性涂层表面上的伪足生长情况。结果表明:稀土梯度生物活性陶瓷涂层碱性磷酸酶(ALP)的表达量均高于TC4合金和未加入Nd2O3的陶瓷涂层,成骨活性较好;成骨细胞向骨细胞分化能力是逐渐增强的;成骨活性与不同含量的Nd2O3合成的HA+β-TCP的数量密切相关,当Nd2O3的添加量为w(Nd2O3)=0.6%时,具有最佳的成骨性能;生物活性复合涂层不仅能引起细胞的粘附生长,更重要的是能够促进细胞的定向分化,且统计学分析表明ALP含量具有明显的显著性。稀土活性梯度陶瓷涂层材料表面细胞伪足生长更旺盛,具有更好的生物相容性。
The method of broadband laser cladding and gradient design was used to increase the biological activity of the composite coating by adding different content of rare earth oxide Nd2O3. The rare earth gradient bioactive ceramic coating containing HA + β-TCP was prepared on the surface of TC4 titanium alloy . MG63 human osteoblasts were co-cultured with Nd2O3 gradient coating in vitro. The content of alkaline phosphatase in osteoblasts was determined by MTT assay and the growth of MG63 cells on the surface of the active coating was observed by SEM. The results showed that alkaline phosphatase (ALP) of rare earth gradient bioactive ceramic coating was higher than that of TC4 alloy and ceramic coating without Nd2O3, and osteogenic activity was better. Osteoblasts differentiated into osteoblasts gradually Enhanced; osteogenic activity is closely related to the amount of HA + β-TCP synthesized with different amounts of Nd2O3, and has the best osteogenic properties when the amount of Nd2O3 added is 0.6% (Nd2O3); the bioactive composite coating Layers not only cause cell adhesion growth, more importantly, it can promote the directional differentiation of cells, and statistical analysis shows that the ALP content has obvious significance. Rare earth active gradient ceramic coating material surface cell pseudopods grow more vigorous, with better biocompatibility.