论文部分内容阅读
肝脏急性反应主要由枯否细胞和肝细胞参与,在急性期反应过程中,枯否细胞将释放大量的细胞因子,肝细胞合成、分泌急性期反应蛋白。本实验拟采用细胞分离、培养技术,了解急性梗阻性胆道感染后肝脏急性期反应与预后的关系;以及Anti-TNF单抗对肝急性期反应的影响。 Wistar大白鼠随机分为两组,即:急性梗阻性胆道感染(AOC)组;Anti-TNF单抗治疗组。两组动物均在术后不同时相麻醉,分离出枯否细胞与肝细胞进行共同培养,测量其上清中TNF、IL-1、IL-6以及CRP、HP的含量变化。
Acute liver reactions are mainly involved in Kupffer cells and hepatocytes. During the acute phase, Kupffer cells release a large amount of cytokines, and hepatocytes synthesize and secrete acute-phase reaction proteins. This experiment intends to use cell isolation, culture technology to understand the acute obstructive biliary tract infection after acute liver response and prognosis; and Anti-TNF monoclonal antibody response to acute liver disease. Wistar rats were randomly divided into two groups: Acute obstructive biliary tract infection (AOC) group; Anti-TNF monoclonal antibody treatment group. Both groups of animals were anesthetized at different phases after operation. Kupffer cells and hepatocytes were isolated and co-cultured. The contents of TNF, IL-1, IL-6, CRP and HP in the supernatant were measured.