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目的 HRP示踪结合Calbindin双标猫外侧膝状体核(LGN)至视皮质的中继神经元.方法 在猫视皮质的17区多点微量注射30%HRP,逆行标记LGN至视皮质的中继神经元,先以金标抗HRP-抗孵育切片,用免疫金银法将HRP颗粒转化黑色银颗粒.然后用ABC法作Calbindin(CB)的免疫组化,试图双标记LGN的中继神经元.结果 HRP标记细胞与CB免疫阳性细胞清晰可辨,HRP标记细胞内为银染黑色颗粒,而CB免疫阳性细胞为染色均匀的棕色.猫LGN的A、A_1和C板层均有CB免疫阳性神经元的分布;HRP标记细胞分布于A和A_1板层.LGN内未见HRP和CB的双标记神经元.结论 LGN内含CB神经元可能不参与视觉信息的传导,而是与局部视觉信息的整合有关.
Objective HRP tracing combined with relaying neurons of the lateral geniculate nucleus (LGN) to visual cortex of Calbindin double-labeled cat.Methods Microinjection of 30% HRP into the visual cortex of the cat’s visual cortex in area 17 and retrograde labeling of LGN to visual cortex Following the neurons, HRP particles were first incubated with anti-HRP-anti-incubated anti-HRP antibody and HRP particles were transformed into black silver particles by immunogold-silver method, then ABC method was used for immunohistochemistry of Calbindin (CB) to try to double labeled LGN’s relay neurons. Results HRP-labeled cells and CB-immunopositive cells were clearly distinguishable, HRP-labeled cells were silver-stained black particles, and CB-immunopositive cells were uniformly stained brown. The HRP-labeled cells were distributed on the A and A-1 plates, and no double labeled neurons of HRP and CB were found in the LGN.Conclusion The CBN neurons in LGN may not participate in the transmission of visual information, but may be associated with the local visual information Integration related.