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甘油醛-3-磷酸脱氢酶(GAPDH)是糖代谢中的核心酶之一,首次完成了水稻中编码此酶cDNA1325bp的全顺序分析,它含有1014bp组成的完整的编码区,编码一个由337个氨基酸组成的肽链。与来源于其他77个物种的GAPDH的同源性比较显示基因间核苷酸同源性高达70％。与美洲龙虾GAPDH三维结构比较说明水稻GAPDH有高度保守性可形成相似的构象,并模建了水稻GAPDH的空间结构。此外,对17种不同进化类型生物GAPDH的氨基酸顺序和22种不同植物GAPDH的核苷酸顺序做了进化关系的分析,并分别画出了系统发生树。结果表明GAPDH是一种适合于分子进化研究的蛋白质。
Glycoaldehyde-3-phosphate dehydrogenase (GAPDH) is one of the core enzymes in carbohydrate metabolism. For the first time, complete sequence analysis of the 1325 bp cDNA encoding this enzyme in rice, which contains a complete coding region of 1014 bp, encodes a full- A peptide consisting of amino acids. Homology with GAPDH from 77 other species showed up to 70% nucleotide homology between genes. Compared with the three-dimensional structure of GAPDH in American lobster, the GAPDH in rice was highly conserved to form a similar conformation, and the spatial structure of GAPDH in rice was modeled. In addition, phylogenetic tree analysis was made on the amino acid sequence of 17 different evolutionary types of biological GAPDH and the nucleotide sequence of 22 different plant GAPDH. The results show that GAPDH is a suitable protein for molecular evolution studies.