论文部分内容阅读
本文报道碳糊电极在含有组氨酸的HAc-NaAc(pH=4.l)溶液中,于-0.10V电位下富集,发现在-0.45V有一灵敏的还原峰.用循环伏安法对它的电化学性质进行了研究,发现在阳极支没有氧化峰,这是一不可逆电极过程.但可用于组氨酸的测定.用微分脉冲伏安法测定组氨酸的最佳测试条件为:pH=4.l的HAc-NaAc,液体石蜡含量为30%,富集电位0.10V,富集时间60s.在4.0~20μg/mL范围内有良好的线性,其标准偏差为0.21,检出限为1.0μg/mL.其余19种氨基酸不干扰组氨酸的测定,可直接用于氨基酸药物样品的分析.并对组氨酸在碳糊电极表面的反应机理进行了讨论.
This paper reports that the carbon paste electrode in histidine containing HAc-NaAc (pH = 4.l) solution, at -0.10V potential enrichment and found a sensitive reduction peak at -0.45 V. Cyclic voltammetry Its electrochemical properties were studied and found no oxidation peak in the anode branch, which is an irreversible electrode process, but can be used for the determination of histidine. Determination of histidine by differential pulse voltammetry best testing conditions: HAc-NaAc with pH = 4.1, the liquid paraffin content was 30%, the enrichment potential was 0.10V and the enrichment time was 60s.It had a good linearity in the range of 4.0 ~ 20μg / mL with a standard deviation of 0.21 and a detection limit 1.0μg / mL.The other 19 kinds of amino acids do not interfere with the determination of histidine, which can be directly used for the analysis of amino acid drugs.The reaction mechanism of histidine on the surface of carbon paste electrode was also discussed.