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目的：试用酶消化法获取鼠原代牙髓细胞，并研究其形态和生长特性。方法：采用０．５％胰酶－０．１％ＥＤＴＡ酶消化法获取ＳＤ鼠原代牙髓细胞，在倒置显微镜下观察其细胞形态和生长情况。结果：细胞形态主要呈梭形和星形，在６ｄ时开始增殖，１３ｄ细胞数达最高，细胞倍增时间为５８．５ｈ（６ｄ～１３ｄ）。结论：用胰酶－ＥＤＴＡ酶消化法可在短期内获得大量的牙髓细胞。
OBJECTIVE: To test primary rat dental pulp cells by enzymatic digestion and study its morphology and growth characteristics. Methods: Primary rat dental pulp cells were obtained by digestion with 0.5% trypsin-0.1% EDTA and observed under inverted microscope for cell morphology and growth. Results: The cell morphology was mainly fusiform and star-shaped, and began to proliferate at 6 days. The cell number reached the highest at 13 days and the cell doubling time was 58.5 hours (6d-13 days). Conclusion: A large number of dental pulp cells can be obtained in a short time by trypsin-EDTA digestion.