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目的研究动脉粥样硬化性脑梗塞（ＡＣＩ）患者载脂蛋白Ｂ（ａｐｏＢ）基因ＥＣＯＲＩ位点的多态性与血脂水平关系。方法应用聚合酶链式反应（ＰＣＲ）－限制性片段长度多态性（ＲＦＬＰｓ）技术，检测８５例ＡＣＩ患者和５６名正常人（ＮＣ）的ａｐｏＢ基因及血脂。结果（１）ＡＣＩ组与ＮＣ组间等位基因频率分布差异无显著意义。（２）ＡＣＩ和ＮＣ组的少见基因型（Ｅ＋Ｅ－或Ｅ－Ｅ－）者血浆甘油三脂（ＴＧ）水平明显高于其Ｅ＋Ｅ＋基因型者（Ｐ＜０．０２）。（３）ＡＣＩ组ＥＣＯＲＩ位点不同基因型个体，总胆固醇（ＴＣ）、ＴＧ水平明显高于ＮＣ组，而ａｐｏＡ水平明显低于ＮＣ组，差异有统计学意义。结论ａｐｏＢ基因ＥＣＯＲＩ位点的ＲＦＬＰｓ对血浆的ＴＧ水平有一定影响。
Objective To investigate the relationship between the polymorphism of the ECORI locus of apoB gene and serum lipids in patients with atherosclerotic cerebral infarction (ACI). Methods Polymerase chain reaction (PCR) - restriction fragment length polymorphism (RFLPs) was used to detect apoB gene and lipids in 85 patients with ACI and 56 normal controls (NC). Results (1) There was no significant difference in allele frequency distribution between ACI group and NC group. (2) Plasma triglyceride (TG) level was significantly higher in rare genotypes (E + E- or E-E-) in ACI and NC groups than in E + E + genotypes (P <0.02). (3) Individuals with different genotypes of ECORI in ACI group had significantly higher level of total cholesterol (TC) and TG compared with NC group and apoA level was significantly lower than NC group. The difference was statistically significant. Conclusion RFLPs at the ECORI site of apoB gene may have some influence on TG level in plasma.