论文部分内容阅读
【目的】探讨附睾分泌蛋白-4(human epididymis protein 4,HE4)和糖类抗原125(cancer antigen125, CA125)在卵巢癌腹水中的表达及临床意义。【方法】收集上皮性卵巢癌患者51例(卵巢癌组),卵巢交界性肿瘤21例(交界性肿瘤组),对照组15例(包括结核性腹膜炎2例、肝硬化2例,急、慢性盆腔炎11例)、分别采用ELISA 法和放射免疫法检测腹水、血清中 HE4和 CA125蛋白表达。【结果】交界性肿瘤组、卵巢癌组腹水、血清HE4阳性表达均显著高于对照组(P<0.05),交界性肿瘤组血清 HE4阳性表达明显高于卵巢癌组(P <0.05)。在卵巢癌不同组织学类型中,子宫内膜样变、浆液性癌中腹水、血清 HE4阳性表达明显增高。在早期卵巢癌(Ⅰ/Ⅱ期)组,血清中 HE4的敏感度明显高于 CA125(P <0.05)。卵巢癌中,腹水、血清 HE4的特异度(均为100%),明显高于 CA125的特异度(P <0.05)。【结论】在卵巢癌腹水、血清中 HE4蛋白表达上调,与卵巢癌的发生和病理生物学行为关系密切,可成为卵巢癌早期诊断的重要肿瘤标志物；联合测定腹水 HE4和 CA125明显提高良、恶性腹水的鉴别。“,”Objective]To investigate the clinical significance of HE4 protein and cancer antigen125(CA125)in ovarian cancer ascites.[Methods]5 1 cases of epithelial ovarian cancer (the ovarian cancer group),21 cases of the o-varian borderline tumor group and 1 5 cases of the control group (including 2 cases of tuberculous peritonitis,2 ca-ses of liver cirrhosis,1 1 cases of acute and chronic pelvic inflammatory disease)were selected.ELISA and radio-im-munity method were used to detect ascites,serum HE4 and CA125 protein expression.[Results]The positive rates of HE4 protein in ascites and serum of the ovarian borderline tumor group and the ovarian cancer group were sig-nificantly higher in than that in the control group(P <0.05).In ovarian cancer of different histological types,the positive expression of ascites in the endometrium ,the serous carcinoma and HE4 was significantly increased.In the early stage (Ⅰ/Ⅱ)of ovarian cancer,the sensitivity of HE4 in serum was significantly higher than that of CA125 (P <0.05).In ovarian cancer,the specificity of ascites and serum HE4 was 100%,which was significant-ly higher than that of CA125 (P <0.05).[Conclusions]Increased protein expression of HE4 were closely associat-ed with tumorigenesis and pathological behaviors of ovarian cancer,which may be used as specific tumor markers to predict the earlier ovarian cancer.Combination of HE4 and CA125 measurement in ascites has an higher diagnos-tic value in differentiate ovarian benign and malignant tumors.