鹅流感病毒2/96-H_5N_1亚型毒株RNA_(1-3)及RNA_5节段核苷酸全序测定

• 作 者: 郭元吉 王敏 郭俊峰 董婕 张烨
• 机 构: 中国预防医学科学院病毒学研究所,中国预防医学科学院病毒学研究所,中国预防医学科学院病毒学研究所,中国预防医学科学院病毒学研究所,中国预防医学科学院病毒学研究所
• 出 处: 中华实验和临床病毒学杂志
• 发表日期: 1999年03期
• 关键词: RNA_5 H_5N_1 PB_1 PB_2 PA和NpRNA 核苷酸全序列 核苷酸序列 鹅流感 编码蛋白 聚合酶链反应

目的　明确广东鹅流感病毒２９６Ｈ５ Ｎ１ 亚型毒株ＲＮＡ１３ 和ＲＮＡ５ 节段核苷酸全序列及其所编码蛋白的氨基酸序列，以及这些基因节段与香港禽流感病毒１５６９７Ｈ５ Ｎ１ 亚型毒株相应节段间的关系。方法　病毒粒ＲＮＡ经逆转录合成ｃＤＮＡ，经聚合酶链反应（ＰＣＲ） 扩增，产物纯化，采用双脱氧链末端终止法进行核苷酸序列测定。结果　广东鹅流感病毒２９６Ｈ５ Ｎ１ 亚型毒株ＲＮＡ１３ 和ＲＮＡ５ 节段长度分别为２３４１，２ ３４１ ，２ ２３３ 和１５６５ 个核苷酸。它们分别编码ＰＢ２（ 含７５９ 个氨基酸），ＰＢ１（ 含７５７个氨基酸） ，ＰＡ（ 含７１６ 个氨基酸） 和ＮＰ蛋白（ 含４９８ 个核苷酸） 。这些蛋白与香港禽流感病毒１５６９７Ｈ５ Ｎ１ 亚型毒株相应蛋白氨基酸序列的同源性分别为９６－４％ ，９７－２％ ，９７－３ ％ 和９７－０％ 。结论　本毒株ＲＮＡ１３ 和ＲＮＡ５ 节段长度分别为２ ３４１，２ ３４１，２ ２３３ 和１ ５６５ 个核苷酸，它们与香港１５６９７Ｈ５ Ｎ１ 亚型毒株间存在着差异

Objective To determine the complete nucleotide sequence of RNA13 and RNA5 of 296H5 N1 subtype strains of Guangdong goose influenza virus and the amino acid sequences of their encoded proteins,  H5 N1 subtype strains corresponding segments of the relationship. Methods The cDNA of virion was reverse transcribed into cDNA and amplified by polymerase chain reaction (PCR). The product was purified and the nucleotide sequence was determined by dideoxy chain termination method. Results The lengths of RNA13 and RNA5 segments of 2  96H5 N1 subtype strains from Guangdong goose were 2341, 2341, 2233 and 1565 nucleotides, respectively. They encode PB2 (759 amino acids), PB1 (757 amino acids), PA (716 amino acids) and NP protein (498 nucleotides), respectively. The homologies of these proteins to the amino acid sequences of the corresponding proteins of the 15697H5 N1 subtype of the Hong Kong bird flu were 96-4%, 97-2%, 97-3% and 97-0%, respectively. Conclusion The RNA13 and RNA5 segments of this strain were 2 341, 341, 233 and 1 565 nucleotides in length, respectively, which were different from the 156  97H5 N1 subtype in Hong Kong