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目的探讨趋化因子受体7(CXCR7)在不同分化程度的胃癌细胞系中的表达以及siRNA沉默CXCR7后对胃癌SGC-7901细胞迁移及侵袭能力的影响。方法体外培养5种人胃癌细胞系:未分化腺癌HGC-27、低分化黏液腺癌MGC-803、低分化腺癌BGC-823、中分化腺癌SGC-7901和高分化腺癌MKN-28。采用Western blot及RT-PCR法分别检测CXCR7的蛋白及mRNA表达。应用脂质体转染siRNA的方法沉默SGC-7901细胞CXCR7的表达,并采用其配体基质细胞衍生因子-1(SDF-1)干预,实验分为4组:阴性对照siRNA(NC siRNA组),NC siRNA+SDF-1组,CXCR7siRNA组和CXCR7siRNA+SDF-1组。应用Transwell小室检测细胞迁移及侵袭能力。结果 CXCR7在不同人胃癌细胞系中表达程度不一,其中高分化MKN-28几乎不表达,中分化SGC-7901细胞表达程度最高。与NC siRNA组相比,NC siRNA+SDF-1组的迁移细胞数和侵袭细胞数增加(P<0.05),CXCR7siRNA组的迁移细胞数和侵袭细胞数减少(P<0.05);与NC siRNA+SDF-1组相比,CXCR7siRNA+SDF-1组的迁移细胞数和侵袭细胞数减少(P<0.05)。结论 CXCR7在中分化腺癌细胞系SGC-7901表达程度最高;SGC-7901细胞的迁移和侵袭能力可被SDF-1提升,亦可被CXCR7siRNA抑制。
Objective To investigate the expression of chemokine receptor 7 (CXCR7) in gastric cancer cell lines with different differentiation and the effect of silencing CXCR7 on the migration and invasion of gastric cancer cell line SGC-7901. Methods Five human gastric cancer cell lines were cultured in vitro: undifferentiated adenocarcinoma HGC-27, poorly differentiated mucinous adenocarcinoma MGC-803, poorly differentiated adenocarcinoma BGC-823, moderately differentiated adenocarcinoma SGC-7901 and well-differentiated adenocarcinoma MKN-28 . The protein and mRNA expression of CXCR7 were detected by Western blot and RT-PCR respectively. The expression of CXCR7 in SGC-7901 cells was silenced by liposome-transfected siRNA and the cells were treated with its ligand-derived cell-derived factor-1 (SDF-1). The experiment was divided into 4 groups: negative control siRNA , NC siRNA + SDF-1 group, CXCR7 siRNA group and CXCR7 siRNA + SDF-1 group. Transwell chambers were used to detect cell migration and invasion. Results The expression of CXCR7 in different human gastric cancer cell lines was different. Among them, well-differentiated MKN-28 was almost not expressed, while the highest level of differentiated SGC-7901 cells was observed. Compared with NC siRNA group, the numbers of migrating cells and invasive cells in NC siRNA + SDF-1 group increased (P <0.05), and the number of CXCR7 siRNA transfected cells and invasive cells decreased (P <0.05) Compared with SDF-1 group, CXCR7siRNA + SDF-1 group had less number of migrating cells and invasive cells (P <0.05). Conclusion CXCR7 has the highest expression in SGC-7901 cells. The migration and invasion ability of SGC-7901 cells can be promoted by SDF-1 and inhibited by CXCR7 siRNA.