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目的探究早期系统性给予白介素-10(IL-10)对成年大鼠胫神经损伤后周围性神经病理性疼痛的影响。方法成年雄性SD大鼠随机分为假手术组、胫神经永久性横断伤(即改良型备用性神经损伤,modified spared nerve injury,mSNI)组、IL-10给药的mSNI组和磷酸缓冲盐溶液(PBS)给药的mSNI组,每组各6只。根据各组的设定进行右侧胫神经永久横断或相应假手术,并在术后立即一次性腹腔注射50μg IL-10(0.1mg/mL)或等体积PBS。每组动物分别于术前(0d)及术后4/5d、7/8d、14/15d在后肢足底隐神经和腓肠神经支配皮肤区域进行足底实验、丙酮测试、von Frey hairs测试、针刺实验以检测其疼痛反应。此外,随机选取6只SD大鼠建立坐骨神经分支选择损伤(spared nerve injury,SNI)模型(胫神经和腓总神经联合横断),并在术后8d观察其后肢足跖外翻状态与mSNI组的异同。结果 mSNI组大鼠损伤同侧后肢足跖外翻状态较SNI组明显减轻。在足底实验、丙酮测试、von Frey hairs测试、针刺实验4种测试中,mSNI组大鼠损伤同侧足底的隐神经和腓肠神经支配皮肤区域在术后4/5d均出现明显的病理性疼痛反应,并持续到术后14/15d。而其损伤对侧及假手术动物损伤同侧及对侧均无明显的病理性疼痛反应。IL-10给药的mSNI组大鼠在损伤同侧足底的隐神经和腓肠神经支配皮肤区域的各种病理性疼痛行为反应在术后4~7d均得到明显的缓解,并持续到术后15d,而PBS给药的mSNI组损伤同侧仍持续保持明显的神经病理性疼痛反应。结论成年大鼠胫神经永久性横断是一种有效的周围性神经病理性疼痛模型,早期系统性注射的给药方式可有效发挥IL-10对周围性神经病理性疼痛的抑制作用。
Objective To investigate the effect of early systemic administration of interleukin-10 (IL-10) on peripheral neuropathic pain after tibial nerve injury in adult rats. Methods Adult male SD rats were randomly divided into sham operation group, tibial nerve permanent transection injury (ie, modified spared nerve injury, mSNI) group, IL-10 administration of mSNI group and phosphate buffered saline (PBS) administered mSNI group, 6 mice in each group. According to the settings of each group, the right tibial nerve was permanently transected or corresponding sham operation, and 50 μg of IL-10 (0.1 mg / mL) or equal volume of PBS was intraperitoneally injected immediately after the operation. The plantar test, acetone test and von Frey hairs test were performed on the saphenous nerve of the hind limbs and the sural nerve of the sural nerve respectively in preoperative (0d), 4/5d, 7/8 d and 14/15d postoperatively. Acupuncture experiments to test its pain response. In addition, 6 SD rats were randomly selected to establish a sciatic nerve injury (spared nerve injury, SNI) model (tibial nerve and common peroneal nerve transection), and 8 h after the operation, the hind limb ectasia and mSNI group Similarities and differences. Results Compared with SNI group, the mtDNA of injured m ipsilateral hind limbs in mSNI group was significantly reduced. In the four kinds of tests of plantar test, acetone test, von Frey hairs test and acupuncture test, the area of saphenous nerve and sural nerve innervated skin of ipsilateral plantar in mSNI group showed obvious Pathological pain response, and continued to 14 / 15d after surgery. However, there was no obvious pathological pain response in the ipsilateral and contralateral sides of the injury contralateral and sham animals. IL-10-administered mSNI rats in the injured ipsilateral soles of the saphenous nerve and sural nerve innervation of the skin area of the various pathological pain behavioral response after 4 ~ 7d were significantly relieved, and continued to surgery After 15 days, the ipsilateral mSNI group with PBS administration maintained the obvious neuropathic pain response. Conclusion The permanent transected tibial nerve in adult rats is an effective model of peripheral neuropathic pain. The systemic administration of early injection can effectively inhibit the inhibitory effect of IL-10 on peripheral neuropathic pain.