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目的 研究光性屈光性角膜切削术（ＰＲＫ）后，转移生长因子β受体Ⅱ（ＴＧＦβＲⅡ） 和表皮生长因子受体（ＥＧＦＲ）ｍＲＮＡ 在角膜的表达变化，探讨角膜上皮下混浊（ｈａｚｅ） 形成的发生机制。方法 新西兰白兔１６ 只，随机分成４ 组。其中１２ 只施行ＰＲＫ，分别于术后１ 、２、３ 个月用裂隙灯显微镜观察ｈａｚｅ 形成情况，并用原位核酸分子杂交方法，检测角膜上皮和基质ＴＧＦβＲⅡ和ＥＧＦＲｍＲＮＡ的表达。结果 ＰＲＫ后１ 个月已有ｈａｚｅ 形成，２ 个月时ｈａｚｅ 最明显，术后３ 个月ｈａｚｅ 减轻或消失。正常兔角膜上皮和基质有ＴＧＦβＲⅡ和ＥＧＦＲｍＲＮＡ表达，ＰＲＫ后角膜ＴＧＦβＲⅡ和ＥＧＦＲｍＲＮＡ表达增加，且以术后１、２ 个月表达最明显。ＴＧＦβＲⅡ和ＥＧＦＲｍＲＮＡ表达强弱与形成ｈａｚｅ 的轻重相关。结论 ＴＧＦβＲⅡ和ＥＧＦＲ参与ＰＲＫ后伤口愈合过程，且调节着ｈａｚｅ 的形成和发展
Objective To study the changes of corneal expression of TGFβRⅡ and epidermal growth factor receptor (EGFR) mRNA in cornea after photorefractive keratectomy (PRK) ) Formed by the mechanism of occurrence. Methods Sixteen New Zealand white rabbits were randomly divided into four groups. PRK was performed in 12 of them, haze formation was observed with slit lamp microscopy at 1, 2 and 3 months after operation, and the expression of TGF-βRII and EGFR mRNA in corneal epithelium and stroma was detected by in situ hybridization. Results One month after PRK, haze was formed, haze was the most obvious at 2 months, haze was reduced or disappeared 3 months after operation. Normal rabbits corneal epithelium and matrix have TGF β R Ⅱ and EGFR mRNA expression, PRK corneal TGF β R Ⅱ and EGFR mRNA expression increased, and the expression was most obvious after 1, 2 months. The expression of TGF β R Ⅱ and EGFR mRNA strength and the formation of haze severity related. Conclusion TGF β R Ⅱ and EGFR participate in wound healing process after PRK, and regulate the formation and development of haze