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铃铛子(Anisodus luridus)是西藏特有的托品烷生物碱药源植物.利用农杆菌C58C1(pRiA4)侵染铃铛子不同器官考察发根诱导频率,并考察了7种培养基(1/2MS,MS,1/2B5,White,WPM,B5和N6)对发根生物量和托品烷生物碱质量分数的影响.真叶和茎段的发根诱导频率分别为53.33%和60.00%,子叶不能诱导出发根.PCR表明rolB和rolC基因已整合到发根的基因组中.B5培养基最适于铃铛子发根生物量和托品烷生物碱积累,发根鲜质量和干质量分别达到6.9±0.94g/瓶和0.44±0.06g/瓶;发根中莨菪碱质量分数为3.49±0.13mg/g.B5和1/2B5培养基中发根的东莨菪碱质量分数高于其他的培养基,分别为1.37±0.06mg/g和1.43±0.22mg/g,二者之间差异无统计学意义.在B5培养基中发根总生物碱产量也是最高的,为2.1±0.29mg/瓶.研究结果为利用发根规模化生产莨菪碱和东莨菪碱奠定了基础.
Anisodus luridus is an endemic alkaloid alkaloid plant in Tibet. The frequency of hair root induction was investigated by using Agrobacterium tumefaciens C58C1 (pRiA4) to infect different organs of bollworm, and the effects of seven mediums (1 / 2MS, MS, 1 / 2B5, White, WPM, B5 and N6) on the root mass and the alkaloid mass fraction of true alkaloids.The frequency of hair root induction was 53.33% and 60.00% Induced root.The PCR showed that the rolB and rolC genes were integrated into the hairy root genome.B5 medium was the most suitable for the accumulation of hair root biomass and alkaloid alkaloids, the fresh and the dried hair root quality reached 6.9 ± 0.94g / bottle and 0.44 ± 0.06g / bottle.The mass fraction of hyoscyamine in hair root was 3.49 ± 0.13mg / g.The scopolamine content in root of hair was higher in medium of B5 and 1 / 2B5 than that of other medium, which were 1.37 ± 0.06mg / g and 1.43 ± 0.22mg / g respectively, there was no significant difference between the two.The highest alkaloid content of hairy roots in B5 medium was 2.1 ± 0.29mg / The use of hairpin mass production of scopolamine and scopolamine laid the foundation.