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基于阿魏酸对鲁米诺-铁氰化钾化学发光体系的抑制作用,建立了阿魏酸的流动注射抑制化学发光分析法,在优化的实验条件下,阿魏酸浓度在4.0×10-7~4.0×10-6mol/L之间时,与化学发光强度减小值呈现良好线性关系,检出限为2.4×10-7mol/L,对1.0×10-6mol/L阿魏酸的相对标准偏差为1.4%(n=9),将其应用于复方当归注射液中阿魏酸含量的测定,结果令人满意.此外,结合化学发光光谱和紫外吸收光谱对抑制化学发光机理进行了初步探讨.
Based on the inhibitory effect of ferulic acid on the chemiluminescence system of luminol-potassium ferricyanide, a flow injection inhibition chemiluminescence assay for ferulic acid was established. Under optimized experimental conditions, the concentration of ferulic acid was 4.0×10- From 7 to 4.0×10-6 mol/L, there is a good linear relationship with chemiluminescence intensity reduction. The detection limit is 2.4×10-7 mol/L, which is relative to 1.0×10-6 mol/L ferulic acid. The standard deviation was 1.4% (n=9), which was applied to the determination of ferulic acid in compound Angelica injection. The results were satisfactory. In addition, chemiluminescence inhibition and chemiluminescence spectra were preliminary combined with chemiluminescence and ultraviolet absorption spectroscopy. Explore.