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应用基因工程技术，对本室已经克隆的日本血吸虫成虫３２ｋＤａ蛋白分子的ｃＤＮＡ片段进行ＰＣＲ扩增，扩增产物亚克隆入高效真核表达载体ｐＣＤ，构建成功裸露ＤＮＡ疫苗ｐＣＤＳｊ３２。ｐＣＤＳｊ３２在ＢＡＬＢ／ｃ小鼠骨骼肌细胞得到表达。表达产物可被小鼠抗３２ｋＤａ蛋白分子单抗识别。免疫荧光定位显示，表达产物不但存在于细胞浆，而且可结合于胞膜并被分泌至胞外。
The cDNA fragment of 32 kDa protein of Schistosoma japonicum, which has been cloned in our laboratory, was amplified by PCR using gene engineering technology. The amplified product was subcloned into the eukaryotic expression vector pCD to construct a successful naked DNA vaccine pCDSj32. pCDSj32 was expressed in BALB / c mouse skeletal muscle cells. The expression product can be recognized by mouse anti-32 kDa protein Mab. Immunofluorescence localization showed that the expressed product not only existed in the cytoplasm, but also bound to the membrane and was secreted extracellularly.