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目的研究血清ＨＣＶＲＮＡ与抗－ＨＣＶ之间的关系。方法对５０例抗－ＨＣＶ阳性的丙型肝炎病人，用酶标仪测血清抗－ＨＣＶＯＤ值，同时用逆转录－套式－聚合酶链反应（ＲＴ－ｎｅｓｔｅｄ－ＰＣＲ）检测血清ＨＣＶＲＮＡ。结果５０例抗－ＨＣＶ阳性丙型肝炎（ＨＣ）患者中３０例ＨＣＶＲＮＡ阳性，即６０％的ＨＣ病人存在病毒血症；在不同的抗－ＨＣＶＯＤ值范围内ＨＣＶＲＮＡ检出率不等，ＨＣＶＲＮＡ检出率随抗－ＨＣＶＯＤ值增高而增加（Ｐ＜０．０１）。结论①血清抗－ＨＣＶ与血清ＨＣＶＲＮＡ之间有较好的一致性；②抗－ＨＣＶＯＤ检测对ＨＣ的诊断及判断ＨＣ病人是否存在病毒血症具有一定价值。
Objective To study the relationship between serum HCVRNA and anti-HCV. Methods Serum HCV RNA was detected by enzyme-linked immunosorbent assay (ELISA) in 50 anti-HCV positive hepatitis C patients and serum HCV RNA was detected by reverse transcriptase-polymerase chain reaction (RT-nested-PCR). Results Thirty cases of HCV RNA were positive in 50 patients with anti-HCV positive hepatitis C (HC), that is viremia was found in 60% of HC patients. The detection rates of HCV RNA were different among different anti-HCV values The rate increased with the increase of anti-HCV value (P <0.01). Conclusion ① There is a good consistency between serum anti-HCV and serum HCVRNA; ② The anti-HCVOD detection has some value for the diagnosis of HC and for the determination of viremia in HC patients.