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为了探索淀粉样蛋白（β－ＡＰ）对神经细胞毒性作用的抑制剂，应用水母发光蛋白导入法研究了海风藤对淀粉样蛋白第２５个氨基酸到３５个氨基酸片断（β－ＡＰ２５－３５）诱导神经细胞胞浆钙离子升高的影响，并应用细胞形态学、细胞存活率、乳酸脱氢酶（ＬＤＨ）释放、细胞集落等指标观察了海风藤对神经细胞的直接作用。结果显示，海风藤在实验浓度内（２．５ｇ／Ｌ～２５ｇ／Ｌ）对神经细胞无毒性作用；海风藤能抑制β－ＡＰ２５－３５诱导的神经细胞胞浆钙离子升高并随海风藤浓度增加而增强。提示海风藤对神经细胞有保护作用，为海风藤防治Ａｌｚｈｅｉｍｅｒｓｄｉｓｅａｓｅ，ＡＤ）提供了依据。
In order to explore the inhibitory effect of amyloid (β-AP) on neurotoxicity, the induction of 25th amino acid to 35 amino acid amyloid (β-AP25-35) of amphioid was studied using the method of aequorin injection. The effects of cytosolic Ca2+ uptake on nerve cells and the direct effects of V. formosanus on nerve cells were observed using cell morphology, cell viability, lactate dehydrogenase (LDH) release, and cell colony. The results showed that the seaweed in the experimental concentration (2.5g/L ~ 25g/L) has no toxic effect on nerve cells; sea vine can inhibit the β-AP25-35 induced neuronal cytoplasmic calcium ion elevation and with the sea vine Increased concentration increases. It is suggested that the seaweed has a protective effect on nerve cells and provides a basis for the prevention and treatment of Alzheimer sdisease (AD).