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松乳菇(Lactarius deliciosus),别名叫松菌、寒菌,岳阳的群众俗称为茅柴菌,在马尾松林地上群生、傲生。可食、味美。春末夏初和秋末冬初出现。我们于1991年进行了纯种分离和驯化栽培,1992年秋得到了少量子实体,1993年又获得了较多的小子实体,现将结果初报如下: 一、材料和方法 (一)种菇来源 松山林采集和集市上采购。 (二)斜面培养基 植物组织培养中常用的MS基本培养基,除去植物生长素和细胞分裂素,补充3g/l蛋白胨、2g/l酵母浸膏,琼脂2％,作为分离菌丝、母种培养和保藏培养基。 (三)原种培养基 小麦粒在冷水中浸泡24～48小时,淘洗干净、晾干装瓶,高压灭菌2小时。另外也用牛粪粉(约15％)与大米混匀,装瓶加水适量(从瓶口加水前沿达瓶底,面上不积水)。
Mushrooms (Lactarius deliciosus), also known as loose bacteria, cold bacteria, Yueyang masses commonly known as Maozhai bacteria, masson pine forests, proud of Health. Edible, delicious. Spring and early summer early autumn and early winter appear. We conducted purebred separation and domestication cultivation in 1991, obtained a small number of fruiting bodies in the autumn of 1992, and obtained more kiddie bodies in 1993. The results are reported as follows: I. Materials and Methods 1. Sources of Mushrooms Pine forest collection and market purchases. (B) the basic medium of MS medium used in plant tissue culture of bevel medium, removing auxin and cytokinin, supplementing with 3g / l peptone, 2g / l yeast extract and agar 2%, as isolated mycelium, Culture and preservation medium. (C) of the original medium wheat grains soaked in cold water for 24 to 48 hours, panning, drying bottling, autoclaving 2 hours. Also use cow dung powder (about 15%) and rice mix, bottled water amount (bottled water from the bottle up front, no water on the surface).