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用酶联免疫斑点（Ｅｌｉｓｐｏｔ）法计数乙酰胆碱受体（ＡＣｈＲ）及其α－、β－、γ－和δ－４种亚单位的特异性ＩｇＧ抗体分泌细胞，评价重症肌无力（ＭＧ）患者外周血Ｂ细胞免疫应答。与其他神经疾病（ＯＮＤ）组相比，ＭＧ组存在显著高水平的ＡＣｈＲ及其４种亚单位的抗体分泌细胞，ＭＧ组４种ＡＣｈＲ亚单位的抗体分泌细胞平均值为１０／１０５～１５／１０５外周血单个核细胞（ＭＮＣ），ＯＮＤ组为０．６／１０５～１．４／１０５ＭＮＣ（Ｐ＜０．０００１）。ＭＧ组ＡＣｈＲ抗体分泌细胞数总是高于任何一种亚单位抗体分泌细胞数，且对α－亚单位的Ｂ细胞免疫应答占有明显优势，这可能是α－亚单位存在主要免疫源区（ＭＩＲ）的间接证据。
The specific IgG antibody secreting cells of acetylcholine receptor (AChR) and its α-, β-, γ- and δ-4 subunits were counted by Elispot method to evaluate the peripheral performance of patients with myasthenia gravis Blood B cell immune response. Compared with other neuropathy (OND) groups, there was a significantly higher level of AChR and four subunit antibody secreting cells in MG group. The average number of antibody secreting cells in MG subtype was 10 / 105-15 / 105 peripheral blood mononuclear cells (MNC), OND group 0.6 / 105 ~ 1.4 / 105MNC (P <0.0001). The number of AChR antibody secreting cells in MG group was always higher than that of any one of the subunit antibody-secreting cells, and the B-cell immune response to the α-subunit had obvious advantages, which may be the main immunogenic region of the α-subunit (MIR ) Indirect evidence.