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研究了ＲＡ对ＳＡＣＩＩＢ２克隆细胞的促分化作用。结果表明，受ＲＡ处理后，ＳＡＣ－ＩＩＢ２克隆细胞形态的改变及生长抑制不因培养液中去除ＲＡ而立即消失，瘤细胞酸性非特异性酯酶（ＡＮＡＥ）染色阳性率明显升高，细胞内嘌呤核苷磷酸化酶（ＰＮＰ）活性明显升高，而腺苷脱氨酶（ＡＤＡ）活性明显降低，末端脱氧核苷酰转移酶（ＴｄＴ）的表达有所减弱，而ＲＡ处理前后瘤细胞乳酸脱氢酶（ＬＤＨ）同工酶谱型无明显改变，提示ＲＡ对ＳＡＣＩＩＢ２克隆细胞有一定程度的诱导分化作用。对ＲＡ处理前后ＳＡＣＩＩＢ２，细胞３－ｆｏｓ基因表达情况也作了初步观察和分析。
The promoting effect of RA on SACIIB2 clone cells was studied. The results showed that the morphological changes and growth inhibition of SAC-IIB2 clone did not disappear immediately after RA treatment, and the positive rate of ANAE staining of tumor cells was significantly increased. The intracellular purine The activity of nucleoside phosphorylase (PNP) was significantly increased, while the activity of adenosine deaminase (ADA) was significantly decreased and the expression of terminal deoxynucleotidyl transferase (TdT) was weakened. However, There was no significant change in the pattern of hydrogenase (LDH) isozymes, suggesting that RA may induce the differentiation of SACIIB2 cells to a certain extent. Before and after RA treatment SACIIB2, cell 3-fos gene expression was also initially observed and analyzed.