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目的 研究细胞粘附分子 CD44变异型 (CD44 v)在食管癌中的表达 ,探讨 CD44变异型表达与食管癌侵袭转移的关系。方法 采用免疫组织化学 L SAB技术 ,检测 80例人食管癌手术切除标本变异型 CD44 v5、CD44 v6、CD44 v7和CD44 v10蛋白表达 ,分析其表达与癌侵袭转移的关系。结果 CD44 v蛋白表达与患者的年龄、性别、食管癌病理分型无明显关系。在正常食管鳞状上皮 ,CD44 v5、CD44 v6、CD44 v7和 CD44 v10在上皮的基底层细胞及近基底层细胞均见表达 ,浅表层上皮表达减弱或阴性 ;在癌组织中 ,与 CD44 v5、CD44 v6相比 ,CD44 v7蛋白表达有非常显著性差异 (P <0 .0 1) ,CD44 v10蛋白表达有显著性差异 (P<0 .0 5 ) ;在转移癌中 ,CD44 v5、CD44 v6蛋白表达与原发性癌相比无显著差异 ;但 CD44 v7蛋白表达与原发性癌相比有非常显著性差异(P<0 .0 1) ,CD44 v10蛋白表达有显著性差异 (P<0 .0 0 5 )。结论 CD44 v7和 v10蛋白表达阳性率降低与食管癌侵袭转移潜能相关 ,是判断食管癌侵袭转移的标志之一。
Objective To study the expression of cell adhesion molecule CD44 (CD44 v) in esophageal carcinoma and to investigate the relationship between CD44 variant expression and the invasion and metastasis of esophageal cancer. Methods The expression of CD44 v5, CD44 v6, CD44 v7 and CD44 v10 protein in 80 specimens of human esophageal cancer was detected by immunohistochemical L SAB technique. The relationship between the expression and the invasion and metastasis was analyzed. Results There was no significant relationship between the expression of CD44v protein and the patient’s age, sex, pathological type of esophageal cancer. In normal esophageal squamous epithelium, CD44v5, CD44v6, CD44v7, and CD44v10 were expressed in both epithelial basal cells and proximal basal cells. The expression of superficial epithelium was attenuated or negative; in cancer tissues, CD44v5, Compared with CD44 v6, there was a significant difference in the expression of CD44 v7 protein (P < 0.01), and there was a significant difference in the expression of CD44 v10 protein (P <0.05); in metastatic carcinoma, CD44 v5, CD44 v6 There was no significant difference in protein expression compared with primary cancer; however, the expression of CD44 v7 protein was significantly different from that of primary cancer (P<0.01), and the expression of CD44 v10 protein was significantly different (P< 0 .0 0 5 ). Conclusion The decreased expression of CD44 v7 and v10 protein is associated with the invasion and metastasis potential of esophageal cancer, and it is one of the markers of the invasion and metastasis of esophageal cancer.