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对Cry3A毒素和氯菊酯杀虫剂经口注射处理的马铃薯甲虫的前肠、中肠和腿节样本的神经肌肉自发动作电位发射进行了记录。两种化合物均可呈现很典型的神经电生理症状 :在初期阶段 ,它们均引起连放动作电位的发放程度大幅增加 ,而发放间隔期延长 ,且随着中毒加深而拉长。氯菊酯可在腿节样本引起典型高频爆排 ,表现出间隔非常短的静息期特征 ,但Cry3A毒素只在肠道样本中表现上述特征。而且Cry3A毒素可使处理甲虫的呕吐物大幅增加 ,而取食减少。这些结果显示处理虫的肠道神经肌肉系统较腿部神经肌肉系统对Cry3A毒素更敏感 ,Cry3A毒素最初的神经毒性或细胞毒性作用引起肠道活动的紊乱是其昆虫毒性作用的重要机制。
Neuromuscular spontaneous action potential emissions from the foregut, midgut and leg segments of the potato beetle treated with Cry3A toxin and permethrin insecticide were recorded. Both compounds exhibit typical neuroelectrophysiological symptoms: in the early stages they both caused a dramatic increase in the release of the putative action potentials, while the release interval was prolonged and elongated as the poisoning deepened. Permethrin caused typical high-frequency explosions in the knuckle samples, showing very short interval resting characteristics, but Cry3A toxin demonstrated these characteristics only in the intestinal samples. And Cry3A toxin can make beetles vomit sharp increase, while feeding reduced. These results show that the intestinal neuromuscular system of the treated worms is more sensitive to Cry3A toxin than the leg neuromuscular system and that the initial neurotoxic or cytotoxic effects of Cry3A toxin cause the disorder of intestinal activity to be an important mechanism of its insect toxicity.