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目的 观察九节龙皂甙 (Ardiusilloside)对人宫颈癌 Hela细胞的抑制作用 .方法 九节龙皂甙 6 .2 5 mg· L- 1 处理 Hela细胞 0～ 72 h,在不同时间点采用光镜观察 Hela细胞形态 ,以琼脂糖凝胶电泳检测 Hela细胞凋亡 DNA的梯形带 ;应用特异性 Ca2 +荧光探测剂 Fluo- 3/ AM在 L SCM条件下检测不同时期细胞内 Ca2 +分布情况 .结果 光镜下体外培养的 Hela细胞在九节龙皂甙作用 2 4h开始出现细胞质皱缩和细胞核凝缩等凋亡的形态学特征 .6 0 ,72 h均检测出 DNA梯形带等凋亡的生物化学特征 .诱导 Hela细胞 48,6 0 ,72 h内 Ca2 +严重超载且维持在较高浓度水平 .结论 九节龙皂甙对体外培养的 Hela细胞具有明显的抑制作用且诱导 Hela细胞凋亡发生 ;细胞凋亡时细胞内 [Ca2 +]增加且维持在较高水平 .
Objective To observe the inhibitory effect of Ardiusilloside on human cervical cancer Hela cells. METHODS: Hela cells were treated with N. scutellariae 6.25 mg·L-1 for 0-72 h. Light microscope was used to observe Hela at different time points. The morphology of cells was detected by agarose gel electrophoresis. The trapezoidal bands of apoptotic DNA were detected by agarose gel electrophoresis. The Ca2 + distribution in the cells at different stages was detected under the condition of L SCM using a specific Ca2 + fluorescent probe Fluo-3/AM. In vitro cultured Hela cells began to exhibit morphological features such as cytoplasm shrinkage and nucleus condensation at 24 hours after the action of saponins. The biochemical characteristics of DNA ladders and other apoptosis were detected at 60 and 72 h. In Hela cells, Ca2 + was overloaded for 48 h, 60 h, and 72 h, and maintained at higher concentrations. Conclusion N. japonicum has significant inhibitory effect on Hela cells cultured in vitro and induced Hela cell apoptosis; apoptosis When the intracellular [Ca2 +] increased and maintained at a high level.