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目的：观察不同声强的８Ｈｚ次声长时间作用后小鼠学习能力的变化并探索其作用机理．方法：学习能力相近的ＢＡＬＢ／ｃ小鼠５０只，随机分５组，除对照组外分别接受９０ｄＢ、１００ｄＢ、１１０ｄＢ、１２０ｄＢ８Ｈｚ次声的作用，每日３ｈ，１４ｄ后用Ｙ－型电迷宫进行学习能力的比较、脑皮质超微结构的电镜观察和脑生化物质的１Ｈ－核磁波谱分析．结果：实验组学习能力均较对照组降低（Ｐ＜０．０５或Ｐ＜０．０１）；电镜观察脑皮质神经细胞出现脂褐素增多及线粒体肿胀．１Ｈ－核磁波谱分析发现乙酰门冬氨酸（ＮＡＡ）、胆碱（Ｃｈｏ）、谷氨酸（Ｇｌｕ）等相对含量显著变化（Ｐ＜０．０１）．结论：９０ｄＢ次声长时间作用即可引起学习能力降低，其原因与次声致使脂质过氧化有关．在９０ｄＢ组和１００ｄＢ组，神经细胞对次声的损害存在代偿反应．
OBJECTIVE: To observe the change of learning ability of mice under 8 Hz infrasound with different sound intensity and to explore its mechanism. Methods: 50 BALB / c mice with similar learning ability were randomly divided into 5 groups. The control group received 90Hz, 100dB, 110dB and 120dB of 8Hz infrasound respectively. The rats were treated with Y-shaped electrical maze for 3h and 14d respectively Comparison of learning ability, electron microscopic observation of cerebral cortex ultrastructure and 1H-NMR spectra of brain biochemical substances. Results: The learning ability of the experimental group was lower than that of the control group (P <0.05 or P <0.01). The increase of lipofuscin and mitochondrial swelling in the cortical neurons were observed by electron microscopy. The results of 1H-NMR showed that the relative contents of NAA, Cho and Glu were significantly changed (P <0.01). Conclusion: 90dB infrasound time can cause reduced ability to learn, the reason and infrasound caused by lipid peroxidation. In the 90dB group and the 100dB group, there is a compensatory response of the nerve cells to infrasound damage.