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作者研究了黑茶藨Ribes nigrum L.果实粗提取物抗流感病毒A型(IVA)和B型(IVB)的活性及其对该病毒在细胞内生长的不同阶段的抑制作用。将感染IVA和IVB的融合的MDCK细胞单层与含1、10和100 μg/mL提取物的最低必需介质培养2天后计算形成的空斑数;还采用空斑形成试验测定了提取物对病毒感
The authors studied the activity of crude extracts of Ribes nigrum L. fruit against influenza virus type I (IVA) and type B (IVB) and their inhibitory effects on different stages of the virus’s growth in cells. The number of plaques formed was calculated after incubation of a monolayer of fused MDCK cells infected with IVA and IVB with the minimum necessary medium containing 1, 10, and 100 μg/mL extracts for 2 days; the plaque-forming assay was also used to determine the extract vs. virus sense