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钴６０Ｘ－射线超大剂量曝射造成Ｃ５７纯种小鼠造血功能抑制。照射３ｄ后，小鼠的外周血白细胞（ＷＢＣ）下降到正常值的２９．１％，８ｄ降至最低，为正常值的１８．６％。骨髓成髓细胞ＤＮＡ含量照射后第３ｄ降至最低，为正常的１８％。国产重组人单核－巨噬细胞集落刺激因子（ｒｈＧＭ－ＣＳＦ）和进口重组人粒细胞集落刺激因子（ｒｈＧ－ＣＳＦ）均有明显升高粒细胞的保护作用。治疗组放疗后立即皮下注射两种造血因子（ｒｈＣＳＦｓ），其对造血干细胞动员作用模式与对照一致，但其升高ＷＢＣ的保护作用非常明显地高于对照组（Ｐ≤０．０１）；预防组共注射ｒｈＣＳＦｓ５ｄ，在注药３ｄ后照射，照射３ｄ后ＷＢＣ降至正常值的约３０％后即持续上升；成髓细胞ＤＮＡ含量ｒｈＧ－ＣＳＦ组降至正常值的３６％及ｒｈＧＭ－ＣＳＦ组降至正常值的３２％，随后持续上升。两种造血因子作用无显著性差异（Ｐ＞０．２）。小鼠试验表明ｒｈＧＭ－ＣＳＦ与ｒｈＧ－ＣＳＦ皮下给予１００与３０μｇ／ｋｇ均呈良好的量效关系。
Overexposure of Cobalt 60X-rays caused hematopoietic suppression in C57-pure mice. After 3 days of irradiation, the peripheral blood leukocytes (WBC) in mice decreased to 29.1% of the normal value and dropped to the lowest level on the 8th day, which was 18.6% of the normal value. The DNA content of bone marrow myeloblasts decreased to the lowest level on the 3rd day after irradiation, which was normal 18%. Domestic recombinant human monocyte-macrophage colony-stimulating factor (rhGM-CSF) and imported recombinant human granulocyte colony-stimulating factor (rhG-CSF) have significantly increased the protective effect of granulocytes. In the treatment group, two kinds of hematopoietic factors (rhCSFs) were injected subcutaneously immediately after radiotherapy. The mobilization mode of hematopoietic stem cells was the same as that of the control group. However, the protective effect of elevated WBC was significantly higher than that of the control group (P≤0.01) The rhCSFs5d group were injected with rhCSFs5d for 3 days, and then the WBC decreased to about 30% of the normal value. The DNA content of myeloblasts decreased to 36% of the normal value and rhGM-CSF group Dropped to 32% of normal, then continued to rise. There was no significant difference between the two hematopoietic factors (P> 0.2). Mice experiments showed that rhGM-CSF and rhG-CSF subcutaneous administration of 100 and 30μg / kg showed a good dose-response relationship.