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目的研究云南恶性疟原虫氯喹抗药性基因(pfcrt)76位点突变的情况,以及与抗药性表现型的关系。方法应用PCR和限制性酶切片段长度分析方法,检测现症病人干滤纸血样的恶性疟原虫pfcrt基因点突变。结果云南省恶性疟原虫pfcrt基因76位点的突变型很高,占85.0%(51/60);野生型和混合型较少,分别占8.3%(5/60)和6.7%(4/60)。体内法测定的氯喹抗性和敏感样本均有pfcrt76突变型;体外法测定的17份氯喹抗性样本中,有13份带有pf-crt76突变型。结论云南省恶性疟原虫pfcrt基因氨基酸编码76位点突变频度很高。体内和体外法测定的氯喹抗性表现与pfcrt76突变型有较高的一致性。
Objective To study the mutation of pfcrt at site 76 of Plasmodium falciparum in Yunnan Province and its relationship with resistant phenotypes. Methods PCR and restriction fragment length analysis were used to detect point mutation of pfcrt gene of Plasmodium falciparum in dry filter paper blood samples. Results The pfcrt gene of P. falciparum in Yunnan Province was highly mutated at position 76 (85.0%, 51/60), with less wild type and mixed type, accounting for 8.3% (5/60) and 6.7% (4/60) ). Chloroquine-resistant and sensitive samples tested in vivo had the pfcrt76 mutant; of the 17 chloroquine-resistant samples tested in vitro, 13 contained the pfcrt76 mutant. Conclusion The pfcrt gene of P. falciparum in Yunnan Province has a high frequency of mutation at codon 76. Chloroquine resistance measured in vitro and in vivo showed a higher identity with the pfcrt76 mutant.