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Background Heat shock protein 70 (HSP70) is expressed highly in epithelial tumours associated closely withhuman papillomavirus 16 (HPV16) infections·However, evidence about the direct relationship between HSP70expression and HPVs infections are still lacking·In the present study, we examined the expression of HSP70 inkeratinocytes introduced with HPV16 E6/E7 oncogenes·Methods Stable transfected cells were established by transfection of the plasmids pLXSN16E6/E7 intocultured primary keratinocytes and subsequently selected by plasmid specific selection antibiotic (G418) at therequired concentration·The expression of HSP70 in pLXSN16E6/E7 transfected keratinocytes was determined byWestern blot·The correlation of HSP70 expression and E6/E7 transfection was further confirmed by doublylabelled immunofluorescent staining·Results Compared to non-transfected keratinocytes, there was a significant trend for higher levels of HSP70 inpLXSN16E6/E7 transfected keratinocytes·Doubly labelled immunofluorescent staining experiment showed thatthe co-localization of HPV16 E6/E7 and HSP70 in transfected keratinocytes was observed and increasedexpression of HSP70 was strongly associated with the transfection of HPV16 E6/E7·Conclusions Our studies demonstrated increased levels of HSP70 proteins in keratinocytes stably transfectedby HPV16 E6/E7 oncogenes·It suggests that the expression of HSP70 is modulated by HPV16 E6/E7 proteins,which may be involved in HPV16 E6/E7 induced immortalization·
Background Heat shock protein 70 (HSP70) is highly expressed in epithelial tumors associated closely withhuman papillomavirus 16 (HPV16) infection. However, evidence about the direct relationship between HSP70expression and HPVs infections are still lacking. In the present study, we examined the expression of HSP70 inkeratinocytes introduced with HPV16 E6 / E7 oncogenes · Methods Stable transfected cells were established by transfection of the plasmids pLXSN16E6 / E7 intocultured primary keratinocytes and subsequently selected by plasmid specific selection antibiotic (G418) at therequired concentration · The expression of HSP70 in pLXSN16E6 / E7 transfected keratinocytes was determined by Western blot · The correlation of HSP70 expression and E6 / E7 transfection was further confirmed by doubly labeled immunofluorescent staining · Results Compared to non-transfected keratinocytes, there was a significant trend for higher levels of HSP70 inpLXSN16E6 / E7 transfected keratinocytes · Doubly labelled im munofluorescent staining experiment showed that the co-localization of HPV16 E6 / E7 and HSP70 in transfected keratinocytes was observed and increase in expression of HSP70 was strongly associated with the transfection of HPV16 E6 / E7 Conclusions Our studies demonstrated increased levels of HSP70 proteins in keratinocytes stably transfected by HPV 16 E6 / E7 oncogenes · It suggests that the expression of HSP70 is modulated by HPV16 E6 / E7 proteins, which may be involved in HPV16 E6 / E7 induced immortalization ·