论文部分内容阅读
探讨传染性单核细胞增多症(infectious mononucleosis,IM)患儿外周血中淋巴细胞亚群的分布及临床意义。采用流式细胞术检测131例IM患儿和50例体检健康儿童外周血中淋巴细胞亚群的表达,131例IM患儿中有26例患儿收集到对应的丙种球蛋白(intravenous immunoglobulin,IVIG)治疗后全血,流式细胞术检测淋巴细胞亚群在IM患儿IVIG治疗前后分布比例的差异。结果显示,与健康对照组相比,IM患儿CD3~+T细胞和CD8+T细胞的表达水平明显增高(均P<0.000 1),CD4~+T细胞、NK细胞、B淋巴细胞及CD4~+/CD8+T细胞比值水平明显降低(均P<0.000 1)。IM患儿急性期CD4~+、CD8~+T细胞及CD4~+/CD8~+T细胞比值升高及降低水平与患儿外周血异型淋巴细胞升高的百分率呈现正相关趋势。与IVIG治疗前相比,IM患儿IVIG治疗后外周血CD3~+T细胞和CD8~+T细胞的表达水平有所减低,但仍高于健康对照组(均P<0.01),治疗后CD4~+T细胞、NK细胞、B淋巴细胞及CD4~+/CD8~+T细胞比值水平增高,但其中治疗后CD4~+T细胞、B淋巴细胞及CD4~+/CD8~+T细胞比值水平仍显著低于健康对照组(均P<0.01)。综上所述,检测淋巴细胞亚群对IM患儿的细胞免疫状况评估、辅助诊断和指导治疗具有重要的临床意义。
To investigate the distribution and clinical significance of peripheral blood lymphocyte subsets in children with infectious mononucleosis (IM). Flow cytometry was used to detect the expression of lymphocyte subsets in peripheral blood of 131 children with IM and 50 healthy children. Totally 26 out of 131 children with IM were enrolled in this study. Intravenous immunoglobulin (IVIG ) After treatment of whole blood, flow cytometry lymphocyte subsets in IM children with IVIG treatment before and after the distribution of the difference. The results showed that the expression levels of CD3 + T cells and CD8 + T cells in IM children were significantly higher than those in healthy controls (all P <0.000 1), CD4 ~ + T cells, NK cells, B lymphocytes and CD4 ~ + / CD8 + T cells were significantly decreased (all P <0.000 1). The percentage of CD4 ~ +, CD8 ~ + T cells and CD4 ~ + / CD8 ~ + T cells in acute stage of IM patients were positively correlated with the percentage increase of peripheral blood lymphocytes in children with IM. Compared with that before IVIG treatment, the expression of CD3 + T cells and CD8 + T cells in peripheral blood of IV children after IVIG treatment was decreased, but still higher than that of healthy control group (all P <0.01) The ratio of CD4 ~ + T cells, NK cells, B lymphocytes and CD4 ~ + / CD8 ~ + T cells increased after treatment, but the ratio of CD4 ~ + T cells, B lymphocytes and CD4 ~ + / CD8 ~ Still significantly lower than the healthy control group (all P <0.01). In summary, the detection of lymphocyte subsets in IM children with cellular immune status assessment, diagnosis and guidance of treatment has important clinical significance.